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. Author manuscript; available in PMC: 2010 Feb 16.
Published in final edited form as: Virology. 2008 Mar 5;375(1):94. doi: 10.1016/j.virol.2007.12.044

Figure 3.

Figure 3

Inhibition of NFkB in human foreskin fibroblasts (HFF) does not lead to increased cellular toxicity, increased lytic gene expression and virion production upon de novo infection with KSHV.

(A) HFFs were transduced with retrovirus encoding a degradation-resistant mutant of IκBα (IκBSR). NFκB DNA binding activity in these cells was blocked upon treatment with 10ng/ml TNFα for 2hrs, as compared to those expressing vector alone.

(B) HFFs expressing IκBSR show no increase in cellular toxicity upon de novo latent KSHV infection. HFFs expressing either vector or IκBSR were infected with KSHV for 6hrs, pictures were taken at 66 hours post infection.

(C) No increase in ORF 59 staining was seen in IκBSR HFFs as compared to vector despite extensive latent KSHV infection as determined by staining for LANA (green). Cells were fixed and stained at 66 hours post infection.

(D) Taqman real time PCR ddCT values for ORF 50, ORF 57 and gB (left panel) and PAN promoter values (right panel) show no increase in lytic gene expression and or virion production in IκBSR expressing HFFs. All values were normalized to GAPDH or spike-in DNA levels, and are expressed for each gene in IκBSR HFFs relative to vector HFFs.