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. Author manuscript; available in PMC: 2011 Jan 26.
Published in final edited form as: Circulation. 2010 Jan 11;121(3):410. doi: 10.1161/CIRCULATIONAHA.109.889725

Figure 5.

Figure 5

Biochemical analyses of TPM1κ. A, Thermal denaturation profiles of recombinant TPM1α and TPM1κ proteins monitored by circular dichroism measurements. The curves show the percentage folding vs. temperature and was calculated based on the ellipticity at a wavelength of 222 nm. B, Cosedimentation assay of TPM1α and TPM1κ binding to cardiac actin. C, Cosedimentation assay of TPM1κ binding to actin with and without human cardiac troponin. The mixture and the pellet fractions were analyzed by SDS-PAGE and Coomassie blue staining.