Figure 6.

Structural models of TPM1κ/κ and κ/α isoforms. A and B, Substitutions in the κ isoform which are predicted to alter the stability of the TPM1 coiled coil are highlighted in color. Red and yellow indicate amino acid substitutions that are likely to destabilize the coiled coil, whereas green indicates a mixed effect and cyan indicates putative stabilizing changes. The oval highlights residues that are predicted to form a salt bridge in a κ/κ coiled coil but would be destabilizing in the κ/α isoform. Legend abbreviations are as follows: -HΦ, loss of hydrophobic contact; -SB, loss of salt bridge; +SB, potential gain of a salt bridge.