Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1988 Oct;85(20):7734–7738. doi: 10.1073/pnas.85.20.7734

Complement receptor type three (CD11b/CD18) of human polymorphonuclear leukocytes recognizes fibrinogen.

S D Wright 1, J I Weitz 1, A J Huang 1, S M Levin 1, S C Silverstein 1, J D Loike 1
PMCID: PMC282267  PMID: 2971974

Abstract

Human polymorphonuclear leukocytes (PMN) have previously been shown to bind to aggregates of fibrin and to fibrinogen-coated surfaces. During their interactions with fibrinogen-coated surfaces, PMN make such close contact with the surface that a portion of the secreted elastase activity is protected from macromolecular protease inhibitors in the surrounding medium. Here we show that the receptor on PMN that mediates this interaction is complement receptor type 3 (CR3; CD11b/CD18), a molecule previously identified as a receptor for the complement protein fragment C3bi. Monoclonal antibodies against CR3 that block the binding of C3bi also block the binding of PMN to fibrinogen-coated surfaces and the formation of a protected compartment. The region of fibrinogen recognized by CR3 lies at the carboxyl terminus of the gamma chain, since peptides based on this sequence effectively inhibit the binding of PMN to fibrinogen-coated surfaces. These peptides also block the binding of C3bi-coated erythrocytes to CR3, thus indicating that a single binding site is used for binding both C3bi and fibrinogen. Sequence analysis shows strong structural similarity between this region of fibrinogen and other known ligands of CR3. These studies thus indicate that CR3 functions as a receptor not only for C3bi but also for fibrinogen.

Full text

PDF
7738

Images in this article

7735Image
on p.7735

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altieri D. C., Mannucci P. M., Capitanio A. M. Binding of fibrinogen to human monocytes. J Clin Invest. 1986 Oct;78(4):968–976. doi: 10.1172/JCI112687. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bai Y., Durbin H., Hogg N. Monoclonal antibodies specific for platelet glycoproteins react with human monocytes. Blood. 1984 Jul;64(1):139–146. [PubMed] [Google Scholar]
  3. Barnhart M. I. Importance of neutrophilic leukocytes in the resolution of fibrin. Fed Proc. 1965 Jul-Aug;24(4):846–853. [PubMed] [Google Scholar]
  4. Burckhardt J. J., Anderson W. H., Kearney J. F., Cooper M. D. Human blood monocytes and platelets share a cell surface component. Blood. 1982 Sep;60(3):767–771. [PubMed] [Google Scholar]
  5. Burns G. F., Cosgrove L., Triglia T., Beall J. A., López A. F., Werkmeister J. A., Begley C. G., Haddad A. P., d'Apice A. J., Vadas M. A. The IIb-IIIa glycoprotein complex that mediates platelet aggregation is directly implicated in leukocyte adhesion. Cell. 1986 Apr 25;45(2):269–280. doi: 10.1016/0092-8674(86)90391-0. [DOI] [PubMed] [Google Scholar]
  6. Clemetson K. J., McGregor J. L., McEver R. P., Jacques Y. V., Bainton D. F., Domzig W., Baggiolini M. Absence of platelet membrane glycoproteins IIb/IIIa from monocytes. J Exp Med. 1985 May 1;161(5):972–983. doi: 10.1084/jem.161.5.972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Coller B. S. A new murine monoclonal antibody reports an activation-dependent change in the conformation and/or microenvironment of the platelet glycoprotein IIb/IIIa complex. J Clin Invest. 1985 Jul;76(1):101–108. doi: 10.1172/JCI111931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dahinden C., Galanos C., Fehr J. Granulocyte activation by endotoxin. I. Correlation between adherence and other granulocyte functions, and role of endotoxin structure on biologic activity. J Immunol. 1983 Feb;130(2):857–862. [PubMed] [Google Scholar]
  9. Dana N., Styrt B., Griffin J. D., Todd R. F., 3rd, Klempner M. S., Arnaout M. A. Two functional domains in the phagocyte membrane glycoprotein Mo1 identified with monoclonal antibodies. J Immunol. 1986 Nov 15;137(10):3259–3263. [PubMed] [Google Scholar]
  10. English D., Andersen B. R. Single-step separation of red blood cells. Granulocytes and mononuclear leukocytes on discontinuous density gradients of Ficoll-Hypaque. J Immunol Methods. 1974 Aug;5(3):249–252. doi: 10.1016/0022-1759(74)90109-4. [DOI] [PubMed] [Google Scholar]
  11. Fleit H. B., Wright S. D., Unkeless J. C. Human neutrophil Fc gamma receptor distribution and structure. Proc Natl Acad Sci U S A. 1982 May;79(10):3275–3279. doi: 10.1073/pnas.79.10.3275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hynes R. O. Integrins: a family of cell surface receptors. Cell. 1987 Feb 27;48(4):549–554. doi: 10.1016/0092-8674(87)90233-9. [DOI] [PubMed] [Google Scholar]
  13. Kloczewiak M., Timmons S., Lukas T. J., Hawiger J. Platelet receptor recognition site on human fibrinogen. Synthesis and structure-function relationship of peptides corresponding to the carboxy-terminal segment of the gamma chain. Biochemistry. 1984 Apr 10;23(8):1767–1774. doi: 10.1021/bi00303a028. [DOI] [PubMed] [Google Scholar]
  14. Lam S. C., Plow E. F., Smith M. A., Andrieux A., Ryckwaert J. J., Marguerie G., Ginsberg M. H. Evidence that arginyl-glycyl-aspartate peptides and fibrinogen gamma chain peptides share a common binding site on platelets. J Biol Chem. 1987 Jan 25;262(3):947–950. [PubMed] [Google Scholar]
  15. Letvin N. L., Todd R. F., 3rd, Palley L. S., Schlossman S. F., Griffin J. D. Conservation of myeloid surface antigens on primate granulocytes. Blood. 1983 Feb;61(2):408–410. [PubMed] [Google Scholar]
  16. RIDDLE J. M., BARNHART M. I. ULTRASTRUCTURAL STUDY OF FIBRIN DISSOLUTION VIA EMIGRATED POLYMORPHONUCLEAR NEUTROPHILS. Am J Pathol. 1964 Nov;45:805–823. [PMC free article] [PubMed] [Google Scholar]
  17. Russell D. G., Wright S. D. Complement receptor type 3 (CR3) binds to an Arg-Gly-Asp-containing region of the major surface glycoprotein, gp63, of Leishmania promastigotes. J Exp Med. 1988 Jul 1;168(1):279–292. doi: 10.1084/jem.168.1.279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sherman L. A., Lee J. Specific binding of soluble fibrin to macrophages. J Exp Med. 1977 Jan 1;145(1):76–85. doi: 10.1084/jem.145.1.76. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Weitz J. I., Huang A. J., Landman S. L., Nicholson S. C., Silverstein S. C. Elastase-mediated fibrinogenolysis by chemoattractant-stimulated neutrophils occurs in the presence of physiologic concentrations of antiproteinases. J Exp Med. 1987 Dec 1;166(6):1836–1850. doi: 10.1084/jem.166.6.1836. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Weitz J. I., Landman S. L., Crowley K. A., Birken S., Morgan F. J. Development of an assay for in vivo human neutrophil elastase activity. Increased elastase activity in patients with alpha 1-proteinase inhibitor deficiency. J Clin Invest. 1986 Jul;78(1):155–162. doi: 10.1172/JCI112545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wright S. D., Craigmyle L. S., Silverstein S. C. Fibronectin and serum amyloid P component stimulate C3b- and C3bi-mediated phagocytosis in cultured human monocytes. J Exp Med. 1983 Oct 1;158(4):1338–1343. doi: 10.1084/jem.158.4.1338. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wright S. D., Meyer B. C. Fibronectin receptor of human macrophages recognizes the sequence Arg-Gly-Asp-Ser. J Exp Med. 1985 Aug 1;162(2):762–767. doi: 10.1084/jem.162.2.762. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wright S. D., Meyer B. C. Phorbol esters cause sequential activation and deactivation of complement receptors on polymorphonuclear leukocytes. J Immunol. 1986 Mar 1;136(5):1759–1764. [PubMed] [Google Scholar]
  24. Wright S. D., Rao P. E., Van Voorhis W. C., Craigmyle L. S., Iida K., Talle M. A., Westberg E. F., Goldstein G., Silverstein S. C. Identification of the C3bi receptor of human monocytes and macrophages by using monoclonal antibodies. Proc Natl Acad Sci U S A. 1983 Sep;80(18):5699–5703. doi: 10.1073/pnas.80.18.5699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wright S. D., Reddy P. A., Jong M. T., Erickson B. W. C3bi receptor (complement receptor type 3) recognizes a region of complement protein C3 containing the sequence Arg-Gly-Asp. Proc Natl Acad Sci U S A. 1987 Apr;84(7):1965–1968. doi: 10.1073/pnas.84.7.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wright S. D., Silverstein S. C. Phagocytosing macrophages exclude proteins from the zones of contact with opsonized targets. Nature. 1984 May 24;309(5966):359–361. doi: 10.1038/309359a0. [DOI] [PubMed] [Google Scholar]
  27. Wright S. D., Silverstein S. C. Tumor-promoting phorbol esters stimulate C3b and C3b' receptor-mediated phagocytosis in cultured human monocytes. J Exp Med. 1982 Oct 1;156(4):1149–1164. doi: 10.1084/jem.156.4.1149. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES