Fig. 1.

Effect of daily orally-administered MSeA and MSeC starting from 8 weeks of age on prostate carcinogenesis surrogate indicators (i.e., weight of genitourinary tract [GU] and weight of the dorsolateral prostate [DLP]) of TRAMP mice at 18 weeks and 26 weeks of age. A and B. The weight of (A) GU and (B) DLP at 18 weeks of age. Wild type littermates (Wild8wk) and TRAMP mice at 8 weeks of age (TRAMP8wk) provided baseline data on GU weight in A. N = 10 mice per group, except for the water-treated control TRAMP group (Water18wk) where one mouse with a 2.6 g prostate tumor was excluded. ** and *** indicate different from water-treated TRAMP group at P<0.01 and <0.001. C. The weight of GU of TRAMP mice in each group at 26 weeks of age presented in an ascending order. Extent of synaptophysin-staining by IHC in the prostate tissues/tumors was marked by − (negative), + (less than 5%) and +++ (greater than 20%). D. Immunoblot detection of synaptophysin expression in selected DLP and prostate tumor (T) samples from the different groups. The number in each sample designation corresponds to the animal No. from which the tissue/tumor was taken. E, the weight of DLP of water-, MSeA- or MSeC-treated TRAMP mice at 26-weeks of age. Water-group, N = 8, excluding 2 mice with large tumors; MSeA-group, N = 9, excluding one mouse with a large tumor, MSeC-group, N = 8, excluding one mouse with a large tumor. F. Body weight of TRAMP mice at 18 and 26 weeks of age. N=10 mice per group. ** indicate different from respective water-treated group at P<0.01.