Fig 2.

Effect of daily orally-administered MSeA and MSeC starting from 8 weeks of age on prostate carcinogenesis lesion progression (A), epithelial proliferation index (B and E.a; Ki67 staining), apoptosis (C and E.b; TUNEL staining) and T-antigen transgene expression (D) in the dorsolateral prostate of TRAMP mice at 18 weeks or 26 weeks of age. A. Lesion grade distribution of water-treated (control), MSeA- or MSeC-treated mice at 18 and 26 weeks of age. The highest lesion grade based on H&E staining in the DLP for each mouse was tabulated. N=10 mice per group. LPIN = low grade prostatic intraepithelial neoplasia, HPIN = high grade prostatic intraepithelial neoplasia, WD = well differentiated carcinoma with epithelial glandular features, MD = moderately differentiated carcinoma with epithelial glandular feature. PD = poorly differentiated carcinomas, mostly like synaptophysin expressing NE-carcinomas. B. Representative staining patterns of Ki67 in DLP tissues from water-treated, MSeA- or MSeC-treated TRAMP mice as well as from a wild type mice at 18 weeks of age. (×400). The overall estimated Ki67 indices for the different groups are shown in Ea based on quantification of five to 10 fields of each slide from each mouse. C. Representative staining patterns of TUNEL apoptosis in DLP tissues of water-treated, MSeA- or MSeC-treated TRAMP mice as well as from wild type mice at 18 weeks of age. (×400). The overall estimated TUNEL positive apoptosis indices for the different groups are shown in E.b based on quantification of five to 10 fields of each slide from each mouse. D. Representative staining patterns of T-antigen protein in DLP tissues from water-treated, MSeA- or MSeC-treated TRAMP mice as well as from wild type mice at 18 weeks of age (×400). Note nuclear staining in epithelial cells of TRAMP DLP, and a lack of staining in wild type DLP. * and ** indicate different from respective water-treated group at P<0.05 and <0.01.