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. Author manuscript; available in PMC: 2010 Feb 20.
Published in final edited form as: Virology. 2009 Dec 6;397(2):299–310. doi: 10.1016/j.virol.2009.11.023

Fig. 3.

Fig. 3

(A) Inhibition of methylation by AdOx treatment. EBNA2 was precipitated with the indicated antibodies from B95.8 cell extract treated with the methylation inhibitor AdOx. Precipitated EBNA2 was visualised using the R3 antibody. The IgG heavy (“IgG-h”) and light (“IgG-l”) chains of the antibodies released from the beads were also detected by the secondary peroxidase coupled anti-rat antibody. Co-electrophoresed molecular mass marker proteins (× 10−3 kDa) were, in descending order: phosphorylase B, bovine serum albumin (BSA), ovalbumin (OVA), and carboanhydrase. (B) Only aDMA-EBNA2 is phosphorylated. The different antibodies as indicated were used to precipitate EBNA2 from 32P-labelled B95.8 cell extracts. The bound EBNA2 was analysed by SDS-PAGE and autoradiography. The position of EBNA2 is indicated. Co-electrophoresed 14C-labelled molecular mass marker proteins (× 10−3 Da) were, in descending order: phosphorylase B, bovine serum albumin (BSA), ovalbumin (OVA), and carboanhydrase.