Fig. 4.

Methylated EBNA2 is present in high molecular weight complexes. Native cell extracts were subjected to 5-40% sucrose gradient centrifugation. The fractions obtained were either concentrated by ethanol precipitation and then analysed by Western blot analysis with the indicated antibodies (panels labelled “WB”) or subjected to precipitation followed by Western blot analysis (panel labelled “IP/WB”) with the indicated antibodies. The GST-specific mAb 6G9 (Kremmer, unpublished) was used as a control for the precipitation. The bands at approx. 50 kDa represent the heavy chains of the antibodies used for precipitation that are detected by the secondary peroxidase-coupled anti-rat antibody. Molecular mass marker proteins were described in Figure 2.