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. Author manuscript; available in PMC: 2010 Feb 20.
Published in final edited form as: Virology. 2009 Dec 6;397(2):299–310. doi: 10.1016/j.virol.2009.11.023

Fig.8.

Fig.8

Chromatin immunoprecipitation analysis. Location of the amplicons generated by the C promoter (A) or LMP1 promoter (B) or LMP2A promoter (C) real-time PCR primer sets. Numbers refer to the start of the amplicons generated by the indicated primer sets relative to the transcription start sites. The RBPJκ and PU.1 binding sites are indicated by grey and black boxes, respectively. The LMP1 promoter sequence has been inverted for simplicity and lies in the reverse orientation in the EBV genome. (D) Chromatin was immunoprecipitated from Mutu I (I) or Mutu III cells (III) using the R3 rat mAb and analysed using Cp-specific primers. To allow comparison between antibodies and experiments relative ChIP signals were calculated by expressing the percentage input signal relative to the Mutu III signal obtained with the furthest downstream primer set. Results show the mean +/− standard deviation for at least 3 independent experiments (n) carried out on at least 2 different batches of chromatin (c). (E) Chromatin immunoprecipitations carried out using the aDMA EBNA2 specific (6F12, open bars) and sDMA EBNA2-specific (7D9, black bars) mouse mAbs, analysed using Cp specific primers. (F) Chromatin immunoprecipitations carried out using the R3 rat mAb analysed using LMP1p-specific primers. (G) Chromatin immunoprecipitations carried out using the aDMA-EBNA2 and sDMA EBNA2-specific mouse mAbs analysed using LMP1p specific primers. (H) Chromatin immunoprecipitations carried out using the R3 rat mAb analysed using LMP2Ap-specific primers. (I) Chromatin immunoprecipitations carried out using the aDMA-EBNA2 and sDMA EBNA2-specific mouse mAbs analysed using LMP2Ap specific primers.