Table 6.
Primers used for Real-Time PCR
| Target gene | Forward primer (5'-3') | Reverse primer (5'-3') | Product size (bp) | PCR Efficiency (%) | Correlation coefficient (R2) | Reference |
|---|---|---|---|---|---|---|
| Clostridium coccoides 16S | aaa tga cgg tac ctg act aa | ctt tga gtt tca ttc ttg cga a | 440 | 97,8 | 0,998 | [43] |
| Bifidobacterium 16S | cgc gtc ygg tgt gaa ag | ccc cac atc cag cat cca | 244 | 93,0 | 0,995 | [44] |
| Lactobacillus 16S | agcagtagggaatcttcca | caccgctacacatggag | 341 | 98,6 | 0,998 | [45,46] |
| Bacteroides spp.16Sa | cgg cga aag tcg gac taa ta | acg gag tta gcc gat gct ta | 360 | 100,1 | 0,997 | This study |
| Butyryl-Coenzyme A | gcn gan cat ttc acn tgg aay wsn tgg cay atg | cct gcc ttt gca atr tcn acr aan gc | 530 | 97,5 | 0,965 | [21] |
| V2-V3 16S region (HDA)b | act cct acg gga ggc agc agt | gta tta ccg cgg ctg ctg gca c | 200 | 113,7 | 0,991 | [40] |
aThe bacteroides primer set was designed to amplify a segment of the DNA sequence represented by the highly homologous bands 4-7 in Table 5. bPCR for the HDA primer set was run in parallel for each set of primers for all samples.