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SAP is a 45 Ca-binding protein exposed on the surface of human sperm. Immuno-staining of PVDF membranes used to detect radioiodinated surface proteins (A) and 45Ca-binding proteins (C) by autoradiography confirmed that the 26.5 kDa calcium binding surface protein with a pI of 5.2 (oblique downward arrows) is SAP (B & D).
