Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The majority of SAP molecules bind to the human sperm surface in a calcium-independent fashion. SAP is slowly released from the surface of washed, swim-up harvested human sperm when incubated in a Ca2+-free DMEM medium (A, left panel). The addition of 5 mM EDTA to the medium induced a similar strong discharge of surface bound SAP within minutes (A, right panel). However, most SAP antigens remained attached to the sperm, despite the removal of calcium from the medium (B).
