Figure 5.
[3H]-NPD1/PD1-specific binding to human PMN. (A) Isolated human PMNs were incubated with the indicated concentrations of [3H]-NPD1/PD1 in the presence or absence of 10 µM unlabeled NPD1/PD1. Saturation curve and Scatchard plot (inset) are the average of triplicates and representative of results from two separate donors. (B) Competition for [3H]-PD1 (3nM)-specific binding to isolated human PMN with increased concentrations of PD1, RvE1 or LXA4. Results are the average of triplicates and representative of n=3.