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. Author manuscript; available in PMC: 2010 Feb 16.
Published in final edited form as: Cell Metab. 2008 Jul;8(1):38–48. doi: 10.1016/j.cmet.2008.05.004

Figure 5.

Figure 5

Inhibiting ERK1/2 protects neurons against oxidative stress. Neurons were cultured in 96-well plates (A, D, E) or glass coverslips in 6-well plates (B). 10–14 DIV neurons were pretreated with ERK1/2 inhibitors U0126 or SL327 for 4 hr. Oxidative stress was induced by H2O2 or menadione. Viability was measured by MTT (A) or live/dead assay (B). (C) % live was calculated from the number of live (green) and dead (red) cells. (D, E) ERK1/2 inhibition primarily attenuates necrosis. Neurons were transfected with control or SirT1 siRNA, or incubated with SL327 before exposed to 400 μM H2O2 or 7.5 μM menadione. 24 hrs later culture media were collected for LDH assay and cells were lysed for apoptosis assay. Data represent mean±SEM.