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. 2010 Feb 17;5(2):e9262. doi: 10.1371/journal.pone.0009262

Figure 4. XA21 Is a Glycosylated Protein.

Figure 4

Myc-XA21 protein was immunoprecipitated from Ubi Myc-XA21 rice using anti-Myc antibody. After washing the Myc-XA21 protein bound to anti-Myc antibody-conjugated agarose beads, Myc-XA21 protein was digested with PNGase F for 2 h at 37°C. Immunoprecipitated Myc-XA21 (IPed) was loaded in the first lane. IPed Myc-XA21 was denatured (second lane) and then treated with PNGase F (third lane). The samples were then subjected to SDS-PAGE for western blot analysis with anti-Myc antibody.