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2D 15N-1H LR-HMQC spectrum of AtraPBP1 at pH 4.5 (black) and pH 7.0 (red). The 15Nε2 resonances of H80 and H95 are marked and exhibit large pH-dependent chemical shift changes. Inset shows a plot of 15Nε2 resonance frequency for H80 (black squares) and H95 (open circles) vs. pH.
