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. Author manuscript; available in PMC: 2011 Feb 23.
Published in final edited form as: Biochemistry. 2010 Feb 23;49(7):1507. doi: 10.1021/bi9016846

FIGURE 3.

FIGURE 3

The IHK promoter directs erythroid-specific expression of DsRed. SB-IHK-DsRed single clones, representing low, medium and high levels of cells expressing DsRed were used to track the modulation of DsRed expression, following treatment to induce erythroid differentiation. (A) Fluorescent microscopy of the clones 14, 49, MS27 and MS28 showing the DsRed expression prior to treatment. (B) Flow cytometry of the cell clones with DsRed (vertical axis) and erythroid specific marker, CD235a, (horizontal axis), under normal culture condition (upper panels) or under hemin treatment for 3 days to induce erythroid differentiation (lower panels). At far left is the parental K562 cells stained with mouse isotype antibody-FITC as a negative control, while the individual clones are identified above the FACS panels. (C) The western blot analysis of the cell clones against SB10, γ-globin, and β-actin control, respectively. The clone ID number as well as presence or absence of hemin treatment are indicated above the lanes.