Figure 2. DC subsets in mLN after influenza infection.

(a) Mice were infected intranasally with PR8 and DC subsets were analyzed by flow cytometry on day 7 after infection. Plasmacytoid DCs were initially excluded based on B220 expression and the three major subsets that remained were defined as conventional DCs (cDCs: MHCII^medCD11c^hi), tissue DCs (tDCs: MHCII^hiCD11c^med) and inflammatory DCs (iDCs: MHCII^medCD11c^medLy6c^hi). Each of these populations was further characterized based on the expression of CD8α, CD4, CD103, CD11b and Ly6C.

(b) C57BL/6 mice were infected intranasally with PR8 and CD8α⁺ cDCs, CD4⁺CD8α⁻cDCs, CD8⁻CD4⁻cDCs, CD103⁺CD11b^lo tDCs, CD103⁻CD11b^hi tDCs and iDCs were enumerated by flow cytometry as described in a. Values represent the total numbers (mean ± SD) of each subset calculated at each time point (n = 4–5 mice / time point). Data are representative of three independent experiments.