Figure 4. EDA-A2 induces cell death in HMEC.

A. Expression of XEDAR in HMECs was determined by qRT-PCR and normalized to β-actin as a house-keeping control. PCR reactions were performed in triplicate and the data presented as fold change in target gene expression (Mean ± S.E.), as explained in the Material and Methods section and Figure 1A. B. HMECs were treated with recombinant EDA-A2 (100 ng/ml and 150 ng/ml) for 72 h and cell survival determined by MTS assay as described previously (37).