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Effect of LY294002 on PI3K signaling in GS-2 cells. A, Western blots showing depletion of phospho-4E-BP1 levels in GS-2 following a 48-hr exposure to 50 μM LY294002. Total 4E-BP1 is shown as a loading control. B, WST-1 cell proliferation assay showing a reduction in the proliferation rate over 48 hrs.
