Effect of PI3K inhibition by LY294002 on LDH activity, LDH levels and HIF-1αlevels in GS-2 and MDA-MB-231 cells. A, Vmax of LDH activity in cell lysates, showing a decrease in the activity with PI3K inhibition. B, Western blot analysis revealing decreases in levels of LDHA and HIF-1αafter treatment. GAPDH is shown as a loading control. C, RT-PCR analysis showing a decrease in LDHA gene expression following PI3K inhibition.