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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: FEMS Immunol Med Microbiol. 2009 Oct 7;58(1):138–145. doi: 10.1111/j.1574-695X.2009.00622.x

FIGURE 2. Siderocalin is induced in lung tissue and alveolar macrophages following M.tb infection.

FIGURE 2

Seven to nine week old female C57BL/6J mice were infected with the Erdmann strain of M.tb via aerosol. (A) Lungs were surgically removed 12 and 24 h post-infection and homogenized in TRIZOL to prepare total RNA. (B) Bronchoalveolar lavage fluid was collected at 12 and 24 h after infection and alveolar macrophages prepared. The cells were homogenized in TRIZOL. Siderocalin expression was determined in total lung and alveolar macrophages using qRT-PCR. Gene expression corresponds to the 2−ΔΔCT between siderocalin and a reference gene (36B4). For statistical analysis, the means ± SEM from a single experiment (n=5) for lung and alveolar macrophages are shown. (*) denotes P ≤ 0.05, (**) denotes P ≤ 0.01 using the Student's t test.