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Glycerol gradient centrifugation of NuMRP and MtMRP. Purified NuMRP or MtMRP containing 50–80 ng of the MRP RNA were centrifuged in parallel on a 15%–30% glycerol density-gradient, and fractioned as described in the Material and Methods. RNA isolated from the gradient fractions and the MRP RNA was detected by Northern analysis. (A) MtMRP. (B) NuMRP.
