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Enzyme titration and time course of RNase MRP cleavage by NuMRP and MtMRP. (A) The 32P-labeled mitochondrial substrate was incubated with increasing amounts of MtMRP or NuMRP for 30 min. Enzyme levels were quantitated by the MRP RNA levels, which are indicated. (B) The 32P-labeled mitochondrial substrate was incubated with the MtMRP or NuMRP for increasing amounts of time. The cleavage reactions were analyzed on a 6% polyacrylamide, 7 M urea gel as described in Materials and Methods.
