FIGURE 4.

The 2′,3′-cylic phosphate terminus of U6 snRNA is identified by the cloning scheme. (A) A 1.2-Mb region (chr4: 89,225,071–90,425,071) from the human genome (hg18) is shown, which contains 12 gene models and a U6 snRNA gene (center). (B) Sequencing reads mapping to the 12-kb interval (chr4: 89,819,071–89,831,071) are plotted, with a maximum of 74 reads. The highest signal in the region corresponds to the U6 snRNA gene, whereas several other positions have one or two mapped reads. (C) Magnification of the region surrounding the U6 snRNA gene (120 kb). (D) Magnification of the region surrounding the U6 gene to base pair resolution (chr4: 89,825,011–89,825,131) showing reads mapping to the 3′ terminal base.