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Fragments of specific tRNA subtypes are identified by the cloning scheme. (A) Diagram of a canonical tRNA. The majority of 2′,3′-cyclic phosphate RNAs correspond to cleavage at the positions in black. (B) Compiled sequence reads mapping to tRNAs of the Glu, His, Lys, and Asp families. Positions along the x-axis are relative to tRNA start site.
