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. 2010 Jan 12;102(3):583–593. doi: 10.1038/sj.bjc.6605487

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Copyright © 2010 Cancer Research UK

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Effects of BD on the activation of NF-κB. PANC-1 cells were treated with 3 and 30 μM BD for 2–4 h. (A, B) Western blots of cytoplasmic protein extracts (20 μg per lane) using antibodies specific for IκB-α and phospho-IκB-α. (C) Western blots of nuclear protein extracts (20 μg per lane) using a phospho-NF-κB p65 antibody. (D) RT–PCR analysis for the mRNA expression of NF-κB-regulated genes, bcl-2 and XIAP. ^*P<0.05 and ^**P<0.01 vs vehicle control. The results were obtained from three independent experiments.