Figure 7.

In vivo analysis of the anti-tumour effects of BD. CAPAN-2 cells were xenografted by subcutaneous inoculation into nude mice. (A) Mice were treated with BD daily at the indicated concentrations through i.v. administration for 10 consecutive days. The tumour volume (TV) of each mouse was determined using the formula: TV=4/3πr³; n=4, where ⁺P<0.05 and ⁺⁺P<0.01 comparing 0.375 mg kg^–1 per day vs vehicle control; ^**P<0.01 and ^***P<0.001 comparing 0.75 mg kg^–1 per day vs vehicle control; ^##P<0.01 and ^###P<0.001 comparing 1.5 mg kg^–1 per day vs vehicle control. (B) Effects of BD on tumour xenograft growth in nude mice after 4 days of treatment at the indicated concentrations. The percentage of tumour growth was calculated after 4 days BD treatment; n=4, ^**P<0.01 and ^***P<0.001 vs vehicle control. (C) The body weight of each mouse was measured for 10 days.