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. 2010 Jan 19;102(3):530–540. doi: 10.1038/sj.bjc.6605538

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Copyright © 2010 Cancer Research UK

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Secreted protein acidic and rich in cysteine (SPARC) inhibits medulloblastoma tumour growth in vivo. Medulloblastoma tumour sections from mice injected with Daoy-P, Daoy-EV, Daoy-SP2, Daoy-EV+pcMMP-9 and Daoy-SP2+pcMMP-9 cells were analysed as described in the ‘Materials and Methods’ section. (A) Haematoxylin and eosin (H&E) staining for the tumours: paraffin-embedded sections of tumours were used for immunohistochemical analysis for SPARC, MMP-9, CD-31 and factor-VIII was performed as described in the ‘Materials and Methods’ section. (B) Tumour volume was quantified and the results are presented in pixels. (C) CD31 (red) and factor-VIII (green) positive areas were counted and quantified as per microscopic field and results are represented. Columns, mean of triplicate experiments; bars, s.e.; ^*P<0.01, significant difference from Daoy parental cells. ^**P<0.05, significant difference from Daoy-SP2 cells. The colour reproduction of the figure is available on the html full text version of the paper.