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. 2009 May 1;42(2):149–160. doi: 10.1165/rcmb.2008-0287OC

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Figure 5. — Neutrophils in the alveolar regions of infant mice. Pregnant bi-TG and control dams received doxycycline in drinking water from E0 or E15 until the pups were killed at PN7. (A–H) Immunohistochemical staining for neutrophils. More neutrophils were seen in the alveoli and alveolar walls of bi-TG pups (E, G, H) than in controls (A–D), except in the case of bi-TG pups of bi-TG dams given doxycycline from E0 (F). (I) Neutrophil counts in the lungs of the offspring. Neutrophil infiltration in response to pulmonary hIL-1β production in the infant mice was abrogated in pups of bi-TG given doxycycline from E0. Open bars, control pups; gray bars, bi-TG pups; n ≥ 4 in each group; ***P ≤ 0.001, bi-TG pups versus control pups; ^†††P ≤ 0.001, compared with bi-TG pups of bi-TG dam given doxycycline from E0. Scale bar, 100 μm. NS, not significant.