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. 2009 May 7;42(2):172–180. doi: 10.1165/rcmb.2008-0270OC

Figure 4.

Figure 4.

Tight junction protein expression by cells cultured for 2 or 5 days on different extracellular matrices. Freshly isolated type II cells were cultured on permeable supports coated with laminin (L), type I collagen (C), or fibronectin (F) for 2 or 5 days, and then harvested, resolved by SDS-PAGE, and levels of claudin-3, -4, -5, -7, -18, zonula occludens (ZO)-1, ZO-2, or occludin were determined by immunoblot. A parallel blot was labeled for actin to normalize to total sample protein content. Relative values for each were normalized to cells cultured for 2 days on laminin. Bar graphs reflect the average (±SEM) for triplicate independent measurements. aSignificantly lower than Day 2 cells on laminin (P < 0.04); bsignificantly lower than Day 2 samples on the same matrix (P < 0.04); csignificantly higher than Day 2 samples on the same matrix (P < 0.04); dsignificantly higher than Day 5 cells on either collagen or fibronectin (P < 0.03); esignificantly lower than Day 5 cells on either collagen or fibronectin (P < 0.05); fsignificantly higher than Day 2 cells on fibronectin (P < 0.05); gsignificantly lower than Day 5 cells on collagen (P < 0.05).