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Histone occupancy changes with transcription of 35S RNA. Chr-IP analysis for quantitative evaluation of H2A and H3 histone amounts in the specified strains (♦, WT; ▴, PSW; □, ATU) with anti-H2A (top graph) or anti-H3 (bottom graph) antibodies. Values refer to H2A or H3 enrichment calculated as IP/input ratio normalized to ACT1 in the different regions and different strains.
