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. 2010 Feb;9(2):242–250. doi: 10.1128/EC.00265-09

Fig. 2.

Fig. 2.

Expression analysis of PsGPR11 during life cycle of and infection by P. sojae. (A) Expression of PsGPR11 during asexual development of P. sojae. Using RT-PCR, we detected PsGPR11 expression from vegetative hyphae (Hy), sporulating hyphae (Sp), zoospores (Zo), cysts (Cy), and germinating cysts (Gc). (B) Expression of PsGPR11 during infection of soybean leaves. Mycelium was inoculated on leaves of soybean cultivar Williams during the indicated time course, i.e., 0.5, 1.0, and 2 h postinoculation. In both panel A and panel B, the top panel (RT+) shows amplification with inner primers of PsGPR11 at 30 cycles (RT− indicates the control reactions when reverse transcriptase was omitted to exclude the possibility of DNA contamination), and the bottom panel indicates the amplifications of the P. sojae actin (Act) gene. RT-PCR products were visualized on ethidium bromide-stained gels, and the sizes are indicated on the right.