Fig. 3.

Generation of PsGPR11-silenced mutants. (A) RT-PCR estimation of PsGPR11 gene expression level using vegetative hyphal RNA from wild type and the indicated transformants. RT-PCRs included either primers of PsGPR11 under the normal RT-PCR conditions with (RT+) or without (RT−) reverse transcriptase or primers for actin A (ActA). The PCR product sizes are shown at the right. (B) Genomic PCR screenings were performed using gDNA as template on indicated strains with the combinations of primers HamF and HamR. M, 200-bp marker. WT, wild type P6497; CK, GUS-expressing transformant, used as a positive control; T2, T45, and T88, PsGPR11-silenced transformants. The PCR product sizes are shown at the right.