Fig. 4.

Aberrant sporangial development of PsGPR11-silenced transformants. (A) Sporangia from wild-type strain P6497 (WT), the GUS-expressing transformant (CK), and three silenced transformants (T2, T45, and T88) were treated by cold shock (15°C) for 30 min and then inoculated at room temperature (25°C) for 4 h. Pictures were taken under a microscope. Bar, 20 μm. (B) Percentages of differential sporangia after cold shock followed by incubation at 25°C for 2, 4, and 8 h. Gray bars, nonreleased sporangia; black bars, completely released sporangia; white bars, partially released sporangia. The total number of sporangia is indicated above each column.