Fig. 6.

Cyst germination of PsGPR11-silenced mutants was reduced. (A) Zoospores from wild-type strain P6497 (WT), a GUS-expressing transformant (CK), and three PsGPR11-silenced mutants (T2, T45, and T88) were encysted by being vortexed for 90 s, and cysts were incubated in clarified 5% V8 liquid medium at 25°C. Microscopic pictures were taken 2 h after inoculation. (B) The numbers of germinated cysts and nongerminated cysts were counted under the microscope at 2 h. The ratio of germinated cysts to the total cysts (germinated and nongerminated) was calculated. Statistics were based on a two-tailed t test. Significant differences were at a level of P < 0.01.