Skip to main content
. 2010 Feb;9(2):242–250. doi: 10.1128/EC.00265-09

Fig. 7.

Fig. 7.

Virulence assay for PsGPR11-silenced mutants. (A) Leaflets of 10-day-old soybean plants (cultivar Williams) were inoculated with equal numbers of zoospores in suspension (1,000 zoospores) from P6497 (WT), the GUS-expressing transformant (CK), and three silenced mutants (T2, T45, and T88) at 25°C. Top and bottom panels show the same leaves, except that the bottom panels show leaves after destaining in alcohol. (B) Leaves of 10-day-old soybean plants (cultivar Williams) were inoculated with hyphal plugs from P6497 (WT), the GUS-expressing transformant (CK), and three silenced mutants (T2, T45, and T88). Pictures were taken at 6 dpi. (C) Detailed pictures of germinated cysts on soybean leaves that were inoculated with zoospores and incubated at 25°C for 12 h. The leaves were then put into ethanol to destain the chlorophyll and subsequently into 0.5% Coomassie brilliant blue for 2 min. After the leaves were washed in water for 10 min, the pictures were taken.