Table 1.

Phenotypic characterization of PsGPR11-silenced transformants^e

Characteristic	WT	CK	T2	T45	T88
Hyphal growtha	0.48 ± 0.06	0.42 ± 0.09	0.50 ± 0.08	0.46 ± 0.05	0.47 ± 0.08
Encystment ratiob	10.2 A	9.6 A	81.3 B	82.0 B	80.5 B
Cyst germinationc	48.87 A	48.22 A	14.00 B	14.76 B	16.19 B
Oospore productiond	42	43	43	43	42

^aBased on 5 days of growth on 10% V8 medium.

^bThe swimming zoospores were incubated on glass plates at 25°C for 1 h. Numbers of swimming zoospores and encysted zoospores were counted under the microscope, and the ratio of encysted zoospores to the total number of zoospores (swimming and encysted) was calculated.

^cPercentage of germinated cysts based on counting a minimum of 100 zoospores from each strain.

^dOospore production was determined on 10% V8 medium.

^eWT, wild-type strain P6497; CK, GUS-expressing transformant; T2, T45, and T88, PsGPR11-silenced transformants. Values represent means ± standard deviations, which were calculated in at least three replicates. Values in the same row followed by different uppercase letters are significantly different at P < 0.01.