Fig. 2.

Effect of rev3Δ and srs2Δ mutations on cell cycle progression of cells lacking Mph1. Asynchronous cultures were grown to mid-log phase, fixed, and stained with Sytox green to measure DNA content by FACS. (A) Cell cycle profiles reveal that mph1Δ srs2Δ cells accumulate in G₂/M phase, which is enhanced further by a rev3Δ mutation. (B) Quantification of cell fractions in G₁, S, and G₂/M phases, determined by FACS analysis of three cell cultures for each strain, using FlowJo v8.3.3. Error bars indicate the standard deviations.