Fig. 3.

The G₂/M arrest of mph1Δ srs2Δ cells is suppressed by disrupting homologous recombination or the DNA damage checkpoint. Cell cycle profiles (A) and quantification (B) of the fractions of cells in G₁, S, and G₂/M phases show that the rad51Δ and mec3Δ mutations are equally effective at decreasing cell accumulation in G₂/M, showing an increase in the fraction of cells in G₁. Neither mutation affects the fraction of cells in S phase. The DNA content of Sytox green-stained cells from at least three mid-log-phase cultures of every strain was analyzed by FACS.