Figure 3.

Macrophages typically induce axonal retraction to the caudal end of the lesion which contains GFAP+ and vimentin+ cells by 28 d postlesion. A, Typical outcome of DCC 28 d after injury. Fibers (red) have retracted a considerable distance and rest on GFAP+ (blue) and vimentin+ (purple) cells. Macrophages (green) still remain in the lesion at this time point. B, Inverted grayscale image of superimposed fiber tracings of three sections from the representative animal in A. C, Atypical outcome of DCC 28 d after injury. DexTR fibers associate with vimentin+ cells and remain in the lesion core. D, Inverted grayscale image of superimposed fiber tracings of three sections from the representative animal in C. E, Higher magnification of vimentin (purple) and fibers (red) as seen in C. Scale bars: A–D, 200 μm; E, 50 μm.