Figure 8.

GM6001 prevents NG2+ cells from crossing the inhibitory proteoglycan rim over 5 d in vitro. A, Untreated NG2+ cells (green) 5 DIV cross the inhibitory spot rim (red), denoted by the dotted line. B, GM6001 prevents NG2+ cells (green) from crossing the spot rim, identified by CS56 (red). C, GFAP+ astrocytes (green) do not cross the spot rim (red) over a period of 5 d. D, The lack of astrocytes crossing the rim does not change upon GM6001 treatment. E, Image (16×) of the proteoglycan rim of a spot after 5 d in vitro with NG2+ cells as visualized with CS56. F, Image (16×) of the proteoglycan rim of a spot after 5 d in vitro with NG2+ cells and the broad spectrum MMP inhibitor, GM6001, taken using the same exposure time as E. G, Adult NG2+ cell crossing of the rim is MMP dependent. The Kruskal–Wallis test was used to test overall significance (χ₍₃₎² = 69.621, p < 0.001). The Mann–Whitney U test was used for pairwise comparisons: ^#p < 0.02, **p < 0.001. Scale bars: A–F, 100 μm.