Table 1.
Primers used for real-time RT-PCR
| Symbol | Gene name | Accession no. | Primer sequence | Amplicon size (bp) | MT (°C) |
|---|---|---|---|---|---|
| CLTC | clathrin, heavy polypeptide (Hc) | X55878 | 5'-AGAAACTGCATGGAGGCACAA | 165 | 81 |
| 3'-TGGGGCTGACCATAAACAATG | |||||
| B2M | beta-2-microglobulin | AB021288 | 5'-GCTATCCAGCGTACTCCAAAGA | 102 | 80 |
| 3'-GGATGGATGAAACCCAGACA | |||||
| COL1A1 | collagen, type I, alpha 1 | Z74615 | 5'-CGAAGACATCCCACCAATCAC | 250 | 92 |
| 3'-TCCCTTGGGTCCCTCGAC | |||||
| MKI67 | antigen identified by monoclonal antibody Ki-67 | X65550 | 5'-GCCTGTACGGCTAAAACATGGA | 182 | 82 |
| 3'-TTGAGGAGAGGCAGGGTGAA | |||||
| RUNX2 | runt-related transcription factor 2 | AF001443 | 5'-ACAGTAGATGGACCTCGGGAAC | 82 | 83 |
| 3'-TGAGGCGGTCAGAGAACAAA | |||||
| ALPL | alkaline phosphatase, liver/bone/kidney | NM_000478 | 5'-CCTGGACCTCGTTGACACCT | 136 | 86 |
| 3'-GTCCCCTGGCTCGAAGAGA | |||||
| SPARC | secreted protein, acidic, cysteine-rich (osteonectin) | J03040 | 5'-TGCCACTGAGGGTTCCCA | 211 | 88 |
| 3'-TCGGTTTCCTCTGCACCATC | |||||
| SPP1 | secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) | NM_000582 | 5'-AACGCCGACCAAGGAAAACT | 150 | 84 |
| 3'-GGCCACAGCATCTGGGTATT | |||||
| COL2A1 | Collagen, type II, alpha1 | MN_001844 | 5'-ATGCTGGTCCTCAAGGCAAA | 196 | 93 |
| 3'-CCAGGAAGACCCCTCAGACC | |||||
| CSPCP | Homo sapiens aggrecan | MN_001135 | 5'-CGAGGAGCAGGAGTTTGTCA116 | 87 | |
| 3'-TCTCAAATTGCATGGGGTGT |