A spatial readout is shown for generation of PtdlnsP3 and Ptdlns(3,4)P2 at the plasma membrane in neutrophil-differentiated HL-60 cells. a–f, Time course of cells exposed to PtdlnsP3 (30 μM)–histone (10 μM). g, Unstimulated cells. h, Cells exposed to PtdlnsP3 (30 μM) without histone carrier. i, Cells exposed to Ptdlns(4,5)P2 (30 μM)–histone (10 μM).