Figure 1.
Yield of oncolytic HSV is greater in hypoxic cultured U87 cells than in normoxic U87 cells. U87 cells (7 × 105) were infected with strain F and oncolytic HSV G207 at a multiplicity of infection (MOI) of 0.4 for 48 hours in normoxic and hypoxic conditions, after which the yield of infectious virus was measured in terms of plaque-forming units (pfus) by plaque assay on Vero cells. The results shown are the mean values, with the error bars representing standard deviations (triplicate plates used for each grouping of virus and oxygen concentration) from a representative experiment. The experiment was repeated three times, each time showing similar results.