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. 2008 Oct 28;17(1):51–56. doi: 10.1038/mt.2008.232

Figure 2.

Figure 2

Hypoxia upregulates GADD34, not the M2 subunit of ribonucleotide reductase. U87 cells were cultured in hypoxic or normoxic conditions for 48 hours. (a) mRNA levels were measured under hypoxic conditions as compared to normoxic conditions, by relative quantification real-time RT-PCR. The bar labeled “RR” represents the mRNA levels for the M2 subunit of ribonucleotide reductase, and a similar lack of induction was seen with the M1 subunit of RR. Error bars represent standard deviations. (b) GADD34 and the M2 subunit of ribonucleotide reductase were also assessed by western blot in normoxic and hypoxic U87 cells.