Fig. 7.

Dependence of growth and octanoate incorporation into phospholipid acyl chains on octanoate concentration in the wild type (open circles) and ΔfabH bypass mutant (closed circles) L. lactis strains. Panel A. Cultures were grown in GM17 medium in the presence various concentrations of the short chain fatty acid, octanoate. Growth was monitored as optical density at 600 nm. Panel B. Incorporation of radiolabeled octanoate into the acyl chains of phospholipids of the wild type and ΔfabH bypass mutant. strains Cultures were grown in the presence of variable concentrations of [1-¹⁴C]octanoate until mid-log phase. Phospholipids were extracted as described in Materials and Methods and radiolabel incorporation into the lipids was determined by scintillation counting.