Table 2.
Complementation of E. coli strains carrying mutations in fabB or in both fabB and fabF by expression of L. lactis FabF. The strains were grown overnight at 30°C or 42°C in the presence of 0.02% arabinose.
| Relevant | Growth Temperature °C |
Fatty acid distribution (percent of total by mass) |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Strain | Phenotype | Plasmid | C14:0 | C16:1 | C16:0 | C18:1 | C18:0 | 16:1/18:1 | SFA/UFA | |
| GRT23a | fabB | vector | 37 | 34.8 | ND | 65.2 | ND | ND | n/a | n/a |
| GRT23a | fabB | pRK52 | 37 | 6.2 | 7.7 | 41.3 | 44.8 | ND | 0.2 | 0.9 |
| CY244 | fabF fabB(Ts) | vector | 30 | 10.9 | 38.8 | 42.3 | 7.8 | <0.1 | 5.0 | 1.1 |
| CY244 | fabF fabB(Ts) | pRK52 | 30 | 5.9 | 13.9 | 2.0 | 74.3 | 3.9 | 0.2 | 0.1 |
| CY244b | fabF fabB(Ts) | vector | 42 | 48.4 | 24.9 | 19.0 | 5.4 | 2.2 | 4.6 | 2.3 |
| CY244 | fabF fabB(Ts) | pRK52 | 42 | 11.1 | 29.5 | 25.4 | 49.7 | 2.6 | 0.6 | 0.5 |
a
These cultures were supplemented with 0.02% CFA.
b
This culture ceased growth due to inhibition of fatty acid synthetic ability.