Upper panels: mean increases in tension of the MF1 mouse isolated vas deferens induced by β,γ-methylene ATP in the presence of DMSO (circles) or cannabigerol (triangles). For the construction of log concentration-response curves, β,γ-methylene ATP was first added 30 min (A) after DMSO or 100 nM cannabigerol (n= 6 or 8) or (B) after DMSO or 1 µM cannabigerol (n= 8). The asterisk indicates a significant difference between the contractile response to 10 µM β,γ-methylene ATP in the absence of cannabigerol and the corresponding response in the presence of this cannabinoid (P < 0.05; unpaired t-test). Lower panel: mean increases in tension of the mouse isolated vas deferens induced by 32 µM phenylephrine in the absence or presence of 1 µM cannabigerol. The two mean values are not significantly different (P > 0.05; unpaired t-test). Additions of phenylephrine were made 30 min after DMSO (open columns) or cannabigerol (n= 8). In all panels, mean increases in tension are expressed in grams ± SEM. DMSO, dimethyl sulphoxide.