Figure 1.

Orexin-A-stimulated ³H-AA (A, C) and ³H-oleic acid (B) release. (A and B) OX₁ receptor-expressing CHO cells (CHO-hOX₁) were used. The release was assessed in NaBM in the presence of 2.4 mg/mL BSA (+BSA) or in the absence of BSA (−BSA). (C) Both wild-type CHO cells (CHO-wt) and CHO-hOX₁ cells were used, both in the absence of BSA. CHO-hOX₁ cells were preincubated for 30 min with SB-334867, changed to fresh NaBM containing the SB-334867 and immediately stimulated with orexin-A. ns (not significant), P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001; comparisons in all subfigures are to the corresponding basal in C, significances are only calculated for wild-type CHO cells and CHO-hOX₁ with 10 µM SB-334867.